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Since microdroplets are able to be generated rapidly in large amount and each droplet can be well controlled as an independent micro-cultivator, droplet microfluidic technology can be potentially used in the culture of microorganisms, and provide the microbial culture with high throughput manner. But its application mostly stays in the laboratory-level building and using for scientific research, and the wide use of droplet microfluidics in microbial technology has been limited by the key problems that the operation for microdroplets needs high technical requirements with wide affecting factors and the difficulties in integration of automatic microdroplet instrumentation. In this study, by realizing and integrating the complicated operations of droplet generation, cultivation, detection, splitting, fusion and sorting, we design a miniaturized, fully automated and high-throughput microbial microdroplet culture system (MMC). The MMC can be widely used in microbial growth curve test, laboratory adaptive evolution, single factor and multi-level analysis of microbial culture, metabolite detection and so on, and provide a powerful instrument platform for customized microbial evolution and screening aiming at efficient strain engineering.
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Industrial Development , MicrofluidicsABSTRACT
Objective:To evaluate the clinical effect of self-made Runfu-Qufeng Decoction combined with conventional western medicine in the treatment of chronic eczema with blood deficiency and wind dryness syndrom. Methods:A total of 80 patients in our hospital from January 2017 to October 2018, who met the inclusion criteria, were selected and divided into two groups according to the random table method, 40 in each group. The control group was treated with conventional western medicine, while the research group was treated with self-made Runfu-Qufeng Decoction on the basis of control group. Both of the two groups were treated for 30 consecutive days with 3 months follow-up. The clinical symptom was scored before and after the treatment and the serum interleukin-4 (IL-4) as well asinterferon-γ (INF-γ) were detected by ELISA. The clinical efficacy was evaluated and the recurrence was recored. Results:The total effective rate of research group was 95.0% (38/40), while the control group was 80.0% (32/40). The difference between the two groups was statistically significant ( χ2=4.114, P=0.043). After the treatment, scores of pruritus degree, lesion area, rash morphology and total scores in the research group were significantly lower than those of the control group ( t values were 9.538, 4.383, 7.992, 4.094, all Ps<0.001). After the treatment, the serum IL-4 level (42.05 ± 3.84 ng/L vs. 47.29 ± 4.16 ng/L, t=5.854) of research group was significantly lower than that of the control group ( P<0.01), while the INF-γ (80.13 ± 7.10 ng/L vs. 74.04 ± 6.26ng/L, t=4.069) was significantly higher than that of the control group ( P<0.01). After 3 months of follow-up, the recurrence rate of the control group was 21.9% (7/32) and that of the research group was 2.6% (1/38). The difference between the two groups was statistically significant ( χ2=5.000, P=0.025). Conclusions:The self-made Runfu-Qufeng Decoction combined with conventional western medicine could relieve the clinical symptoms of patients with chronic eczema due to blood deficiency and wind dryness syndrom and improve the clinical efficacy.
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Objective:To evaluate the clinical effect of Qingjin-Huatan Decoction combined with conventional western medicine in the treatment of acute aggravation of bronchiectasis. Methods:A total of 80 patients in Beijing Sixth Hospital from January 2014 to December 2018 were included. Patients were divided into two groups according to random number table method, 40 cases in each group. The control group was treated with conventional western medicine. The research group was treated with Qingjin-Huatan Decoction based on the control group. Both of the two groups were treated continuously for 7 days. Before and after the treatment, their symptoms and signs were scored, forced expiratory volume in one second (FEV1), forced vital capacity (FVC) and peak expiratory flow (PEF) were detected by pulmonary function tester, serum neutrophil elastase (NE) and cathepsin G were detected by ELISA, tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4) and interleukin-6 (IL-6) in sputum were detected by ELISA, and clinical efficacy was evaluated. Results:The total clinical effective rate of the research group and the control group was 90.0% (36/40) and 77.5% (31/40) respectively. The difference between the two groups was statistically significant ( χ2=4.501, P=0.034). After the treatment, the scores of cough, expectoration, phlegm quality, phlegm quantity, fever, shortness of breath, thirst, chest distress and wet rales in the research group were significantly lower than those of the control group ( t values were 15.240, 16.910, 21.761, 18.949, 16.740, 9.285, 11.732, 9.050, 11.193, all Ps<0.001), FEV1, FVC and PEF were all significantly higher than those of the control group ( t values were 5.085, 4.468 and 4.219, respectively, all Ps<0.001), the levels of TNF-α, IL-4 and IL-6 in sputum were all significantly lower than those of control group ( t values were 8.341, 8.884 and 11.352, respectively, all Ps<0.01), and the levels of serum NE (26.20 ± 3.18 μg/L vs. 51.15 ± 5.24 μg/L, t=25.744) and cathepsin G (5.62 ± 1.01 μg/L vs. 8.05 ± 1.33 μg/L, t=9.203) were significantly lower than those of control group ( P<0.001). Conclusions:The Qingjin-Huatan Decoction combined with conventional western medicine can relieve clinical symptoms of patients with acute aggravation of bronchiectasis, improve pulmonary function, inhibit the expression of inflammatory cytokines and proteolysis activities in the airway, and improve the clinical efficacy.
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Objective To analyze the psychological and behavioral status and influencing factors of college students during the epidemic of COVID-19 in Wuhan. Methods The students from two universities in Wuhan area were selected by stratified cluster sampling, and the self-designed questionnaires were used to conduct the online survey. Statistical analysis was performed using SPSS 22.0. Results Among 3 966 college students surveyed in this study, the proportion of students in Wuhan who thought that their own infection probability was higher than others (11.25%), that their family members were more likely to be infected (15.29%), that they were very worried about their own infection (22.93%), that they were very worried about the infections in the same community (25.27%), and that their own infection risk was higher than others (9.77%), was higher than those of students in other three regions. The total incidence of depression was 27.10%. Multivariate logistic regression analysis showed that the humanities and social science majors living in the city, worrying about the infection of themselves and their families, feeling that they had a higher chance of infection than other, being not optimistic about the epidemic situation, and thinking that the infection had a great impact on people's emotions were the risk factors of depression during the epidemic. Conclusion College students in Wuhan had psychological and behavioral problems during the COVID-19 epidemic. These problems were related to whether they were located in the epidemic area and whether they were worried about infections.
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Objective@#To explore the relationship between gross motor development and perceived motor competence among children aged 7-8 years old.@*Methods@#Random cluster sampling method was used to conduct a test among 162 students aged 7-8(78 boys,84 girls)by using Test of Gross Motor Development Ⅲ(TGMD-3) and 3 and Pictorial Scale of Perceived competence and Social Acceptance for Young Children(PSPC-P).@*Results@#There were no statistical significance of perceived motor competence in different genders,and ages among children(t=-0.82, -0.58, P>0.05). Gross motor development differed significantly in different age groups (t=-4.79, P<0.01). Eight-year-old group(63.10±5.70) scored higher than 7-year-old group(57.96±7.76).There exists clear association between gross motor scores and perceived motor competence (r=0.29, P＜0.01), as well as locomotor development and manipulative development with perceived motor competence (r=0.21, 0.24, P<0.05).@*Conclusion@#There are close correlations between gross motor development and perceived motor competence among children aged 7 to 8 years. Manipulative development shows obvious influences on perceived motor competence. No gender differences are found in perceived motor competence.
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Objective To develop a Class Ⅲ twin-block magnetic orthopedic appliance (TMOA-Ⅲ) and to investigate the treatment effects of this appliance on skeletal Class Ⅲ malocclusion of mixed dentition and early permanent dentition.Methods The sample consisted of 76 Chinese children (42 males and 34 females,mean age 9.1 years) with Class Ⅲ malocclusion caused by maxillary retrognathism,and the subjects were divided into a treatment group and a control group.The treatment group,38 subjects (21 males and 17 females),were instructed to wear TMOA-Ⅲ for 5-8 months (mean 7.5 months) and the control group,38 subjects (21 males and 17 females),did not wear any appliance.Cephalometric radiographs were taken and measurement data were used for analysis.Results Compared with the control group,patients of the treatment group showed a favorable increase of maxilla length and anterior movement (PNS-A,P＜0.001;SNA,A-NPg,P＜0.05),and skeletal Class Ⅲ facial profiles improved (UL-EL,NsPgs-HL,Facial convexity,P ＜ 0.001;LL-EL P ＜ 0.005).Conclusions The results indicate that TMOA-Ⅲ is effective for the treatment of Class Ⅲ malocclusion caused by maxillary retrognathism in mixed dentition and early permanent dentition,which provides another choice for the treatment besides the face mask.
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Objective To investigate the predictive effect of hypersensitive C-reactive protein(Hs-CRP)and neutrophil and lymphocyte ratio(NLR)on the prognosis in young patients with ischemic stroke. Methods From October 2016 to October 2017,110 consecutive young patients(18-45 years old)with ischemic stroke admitted to the Department of Neurology,Xuanwu Hospital,Capital Medical University were enrolled retrospectively. According to the modified Rankin scale(mRS)scores,they were divided into either a good prognosis group(mRS≤2;n=90)or a poor prognosis group(mRS>2;n=20).The patients completed the related examinations within 24 h after admission,including blood routine and Hs-CRP.The NLR value was calculated according to the count of neutrophils and lymphocyte in blood routine.The age,gender,underlying diseases(hypertension,diabetes,hyperlipidemia,hyperhomocysteinemia),histories of smoking and drinking,National Institutes of Health stroke scale(NIHSS)scores of both groups of patients were documented.The poor prognosis after discharge at 90 d was used as the dependent variable,the independent variables of P<0.05 in univariate analysis were further performed with multivariate logistic regression analysis.The receiver operating characteristic(ROC)curve was used to evaluate the sensitivity and specificity of the independent risk factors. The Youden index was calculated and the optimal cut-off value was determined. Results (1)Compared with the good prognosis group,the poor prognosis group had higher NIHSS score,NLR and Hs-CRP at admission.The differences between the 2 groups were statistically significant(9.0[4.5,13.0]vs.2.5[2.0,4.0],2.97[2.31,4.20]vs.2.13[1.76,2.70],4.65 [2.70,9.52]mg/L vs.2.06[0.87,4.54]mg/L;all P<0.05).There were no significant differences in other baseline data and clinical characteristics between the two groups(all P>0.05).(2)The results of the multivariate logistic regression analysis indicated that the high level of Hs-CRP(OR,1.086,95%CI 1.009-1.169)and higher NIHSS score(OR,1.487,95%CI 1.229-1.797)at admission were the independent risk factors for poor prognosis(all P < 0. 05),and there was no significant relation between NLR and prognosis(P>0.05).(3)The area under the ROC curve of the Hs-CRP levels at admission was 0.722(95%CI 0.591-0.853,P=0.002).When the predictive value of Hs-CRP level at admission was 3.365 mg/L,the maximum Youden index was 0.367,its corresponding sensitivity was 70.0%and specificity was 66.7%. Conclusions The higher Hs-CRP level and NIHSS score at admission may independently predict the poor prognosis of young patients with ischemic stroke at 90 d after discharge to a certain extent.It is not appropriate to use Hs-CRP≥3.365 mg/L alone for poor prognosis screening,but NLR may not be associated with the prognosis at admission.
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Objective:To study the influence of prophylactic bilateral salpingectomy during hysterectomy in ovarian function of the premenopausal women with benign uterine disease and its preventive effect on the pelvic diseases(malignant /benign pelvic lesions or pelvic inflammatory disease).Methods:A total of 100 patients with benign uterine disease underwent hysterectomy were collected and divided into two groups,including 50 patients underwent simultaneous hysterectomy and prophylactic bilateral salpingectomy (prevention group) and 50 patients underwent only hysterectomy (control group).The operative time,blood loss,evacuation time,and hospitalization time of the patients in two groups were detected;the antral follicle count,levels of serum estradiol(E2),follicle stimulating hormone(FSH) and luteinizing hormone(LH)) and the incidence of perimenopausal symptoms of the patients in two groups before operation and six months,1 year after operation were compared;the incidence of pelvic disease of the patients after operation were compared.Results:The operative time,blood loss,evacuation time,and hospitalization time of the patients between two groups were not significantly different(P>0.05).Compared with before operation,the antral follicle count and the E2 levels of the patients 6 months and 1 year after operation in two groups were decreased,and the FSH and LH levels of the patients were inc reased (P0.05).The incidence of perimenopausal symptoms of the patients in two groups 6 months and 1 year after operation showed no significant difference(P>0.05).The incidence of ovarian malignant tumor and ovarian benign tumor of the patients in two groups 6 months and 1 year after operation had no significantly differences (P>0.05).The incidence of pelvic inflammatory disease of the patients in control group was higher than that in prevention group(P<0.05).There were 2 patients diagnosed as tubal carcinoma in control group 1 year after operation,and the pathological findings showed the atypical cells in 2 patients in prevention group.Conclusion:Prophylactic bilateral salpingectomy during hysterectomy does not damage the ovarian function and can reduce the incidence of pelvic malignant/ benign tumor and pelvic inflammatory disease.
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Objective:To study the influence of metformin and paclitaxel in the proliferation and apoptosis of human ovarian cancer SKOV3 cells in vitro, and to clarify the synergistic effect of metformin and paclitaxel. Methods:The ovarian cancer SKOV3 cells were divided into blank control group, different concentrations (0.01, 0.50, 1.00, 5.00, 10.00mmol·L-1) of metformin groups and combined treatment groups(metformin combined with paclitaxel with different concentrations).The inhibitory rates of proliferation of SKOV3 cells after treated with different concentrations of metformin were detected by MTT method.The apoptotic rates of SKOV3 cells after treated with different concentrations of metformin were measured by flow cytometry.The inhibitory rates of proliferation of SKOV3 cells after treated with metformin and paclitaxel were determined by MTT method.Results:The MTT results showed that the inhibitory rates of proliferation of SKOV3 cells in different concentrations of metformin groups were increased in concentration-and timedependent manner;there were significant differences compared with blank control group (P<0.05).The flow cytometry results showed that the apoptotic rates of SKOV3 cells in different concentrations of metformin groups were increased;compared with control group, with the increasing of concentrations of metformin, the apoptotic rates of SKOV3 cells in experimental groups 48 h after treatment were increased significantly (P<0.05);the percentages of SKOV3 cells in G0/G1 phase were decreased with the increasing of metformin concentrations(P<0.05) and the percentages of SKOV3 cells in G2/M phase were increased(P<0.05).The MTT results showed that the inhibitory rate of proliferation of SKOV3 cells in 1.00 mmol·L-1 metformin+paclitaxel group was higher than that in 0.50 mmol·L-1 metformin+paclitaxel group was higher than that in 0.50 mmol·L-1 metformin+paclitaxel group(P<0.05),and the inhibitory rates of proliferation of SKOV3 cells in combined treatment groups were higher than those in paclitaxel alone groups(P<0.05).Conclusion:Metformin inhibits the proliferation of ovarian cancer SKOV3 cells through the induction of apoptosis.Metformin can enhance the cell proliferation inhibition of paclitaxel on ovarian cancer SKOV3 cells.The combination of metformin and paclitaxel has a synergistic reaction on SKOV3 cells.
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Objective To analyze the characteristics on demographical,spatial distribution and transmission mode of HIV infections among 50-year-old population.Methods Related information on demography,spatial distribution,high risk behavioral and history of HIV infections among 50-year-old population were collected and analyzed.Possible time of infection based on their first CD4 testing results right after the HIV diagnoses,was estimated.Results Since 2008,the number of new HIV/AIDS cases among the over-50-year-olds was reported increasing annually.The number of aged 50 and above in 2014 was 4.2 times than the number in 2008.50-year-old or older population were infected mainly through heterosexual behavior (88.0％).Among these cases,83.9％ self-reported as having histories on extramarital sex intercourse and the number was increasing yearly.Among male cases who admitted as having heterosexual experience,95.1％ of them reported as having histories of extramarital sex intercourse while 53.4％ of the female cases reported as having the same experiences.46.6％ of spouses of the females or with fixed partners were HIV positive.Through estimating the time of infection and the time interval between infection and diagnosis,we found that the proportion was 15.5％,from infection to diagnosis as 3 years among the 50 and older age groups,but the proportion of 8 years from infection to diagnosis was 43.6％.We estimated that 66.5％ of the new HIV cases who were at age 50 and over,were infected when they were at that age span.The average time from infection and being tested was (6.8 ± 2.7) years.Conclusion The increasing number of being diagnosed on HIV among the 50-year-olds population might be related to both high risk exposure and belated diagnoses among this population,calling for the necessity of deriving the sources of HIV infection and tailoring the HIV prevention strategies in this population.
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Objective To analyze the ‘late diagnoses’ (LD) phenomena among newly identified HIV/AIDS cases in China from 2010 to 2014.Methods Newly identified HIV/AIDS cases from 2010 to 2014 were selected from the National HIV/AIDS comprehensive information system.Rates related to LD were stratified according to the demographic indicators.Results From 2010 to 2014,numbers of newly identified HIV/AIDS cases were reported as 64 338,74 517,82 434,90 119 and 103 501 in China.Among them,proportions of LD were 41.8％,42.1％,38.1％,36.8％ and 35.5％ respectively.LD proportions appeared high in medical settings,with the minimum proportion as 45％ (x2=5 174,P＜0.000 1).Proportion of LD among patients who were heterosexually transmitted appeared over 40％,which was significantly higher than that seen among men who have sex with men or injecting drug users (x2=3 066,P＜0.000 1).Proportion of LD showed more than 39％ in those newly identified cases,and was seen higher in permanent residents than in floating population (less than 30％) (x2=5 265,P＜0.000 1).Conclusion With the expansion testing coverage on HIV,newly identified HIV/AIDS increased year by year.Among the newly identified cases,late diagnosis was high,especially in medical institutions.More effective strategies and measures are needed to promote the early detection of HIV in high risk population.
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Objective To analyze the characteristics on demographical,spatial distribution and transmission mode of HIV infections among 50-year-old population.Methods Related information on demography,spatial distribution,high risk behavioral and history of HIV infections among 50-year-old population were collected and analyzed.Possible time of infection based on their first CD4 testing results right after the HIV diagnoses,was estimated.Results Since 2008,the number of new HIV/AIDS cases among the over-50-year-olds was reported increasing annually.The number of aged 50 and above in 2014 was 4.2 times than the number in 2008.50-year-old or older population were infected mainly through heterosexual behavior (88.0％).Among these cases,83.9％ self-reported as having histories on extramarital sex intercourse and the number was increasing yearly.Among male cases who admitted as having heterosexual experience,95.1％ of them reported as having histories of extramarital sex intercourse while 53.4％ of the female cases reported as having the same experiences.46.6％ of spouses of the females or with fixed partners were HIV positive.Through estimating the time of infection and the time interval between infection and diagnosis,we found that the proportion was 15.5％,from infection to diagnosis as 3 years among the 50 and older age groups,but the proportion of 8 years from infection to diagnosis was 43.6％.We estimated that 66.5％ of the new HIV cases who were at age 50 and over,were infected when they were at that age span.The average time from infection and being tested was (6.8 ± 2.7) years.Conclusion The increasing number of being diagnosed on HIV among the 50-year-olds population might be related to both high risk exposure and belated diagnoses among this population,calling for the necessity of deriving the sources of HIV infection and tailoring the HIV prevention strategies in this population.
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Objective To analyze the ‘late diagnoses’ (LD) phenomena among newly identified HIV/AIDS cases in China from 2010 to 2014.Methods Newly identified HIV/AIDS cases from 2010 to 2014 were selected from the National HIV/AIDS comprehensive information system.Rates related to LD were stratified according to the demographic indicators.Results From 2010 to 2014,numbers of newly identified HIV/AIDS cases were reported as 64 338,74 517,82 434,90 119 and 103 501 in China.Among them,proportions of LD were 41.8％,42.1％,38.1％,36.8％ and 35.5％ respectively.LD proportions appeared high in medical settings,with the minimum proportion as 45％ (x2=5 174,P＜0.000 1).Proportion of LD among patients who were heterosexually transmitted appeared over 40％,which was significantly higher than that seen among men who have sex with men or injecting drug users (x2=3 066,P＜0.000 1).Proportion of LD showed more than 39％ in those newly identified cases,and was seen higher in permanent residents than in floating population (less than 30％) (x2=5 265,P＜0.000 1).Conclusion With the expansion testing coverage on HIV,newly identified HIV/AIDS increased year by year.Among the newly identified cases,late diagnosis was high,especially in medical institutions.More effective strategies and measures are needed to promote the early detection of HIV in high risk population.
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Objective To study the pulmonary artery shrink pressure(PASP) and pulmonary function in children with asthmatic diseases,and screen pulmonary arterial hypertension (PAH).To probe the correlation between PASP and pulmonary function.Methods Ninety children with asthmatic diseases from November 2014 to April 2015 at the Department of Respiratory,Women and Children's Hospital of Qingdao City were chosed as asthmatic group,and 90healthy children in the same period were selected as healthy control group.PASP and pulmonary function were detected by Doppler ultrasound and tidal flow-volume loop respectively within 24 hours of admission,the respite degrees were assessed by clinical and tidal flow-volume loop.SPSS 13.0 statistical software was used for statistical analysis.Results PASP of the asthmatic group and the healthy control group was (3.24 ±0.66) kPa and (3.15 ±0.49) kPa,and the difference was statistically significant (F =7.50,P ＜ 0.05).There were 10 cases of PAH in the asthmatic group,which had statistically difference with the healthy control group (x2 =10.59,P ＜ 0.05).The tidal volume per kilogram,the ratio of time to peak tidal expiratory flow to total expiratory time,the ratio of volume to peak expiratory flow to total expiratory volume of the asthmatic group were (6.62 ± 1.63) mL/kg,(26.76 ± 6.48) ％ and (26.66 ± 6.54) ％,and these three indicators of the healthy control group were (8.38 ± 0.80) mL/kg,(34.35 ± 2.84) ％ and (34.23 ±3.02) ％,but that of the former group were less than that of the lower respectively (F =86.28,138.08,113.27,all P ＜0.05).PASP had negative correlation with the ratio of time to peak tidal expiratory fow to total expiratory time in the asthmatic group (r =-9.45,P ＜ 0.05).PASP was increased with the aggravation of respite,and it had statistical difference between the mild or moderate asthmatic group [(3.43 ± 0.50) kPa,(3.66 ± 0.59) kPa] and the severe group [(4.20 ± 0.75) kPa] (x2 =24.81,12.55,all P ＜ 0.05),and the cases of PAH had significant differences among these 3 groups (0,2,8 cases in mild,mode rate and severe group,respectively) (x2 =54.92,P ＜ 0.05).Conclusions For children with more severe airway obstruction and wheezing,the risk of PASP and PAH will be higher.It is helpful to reduce right ventricular function damage for children with asthmatic diseases by measuring PASP and screening PAH in the early stage according to the clinical condition and lung function.
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Objective compare the changes in condyle positions, face type lateral position and facial soft tissue before and after clinical correction of adult ClassⅡ Division Ⅰ malocclusion patients accompanied with the temporomandibular disorder. Method there were 7 adult Class Ⅱ Division Ⅰmalocclusion patients with accompanied with temporomandibular disorder, they accepted orthodontic extraction treatment routinely with MBT straight wire appliance technology, by which the occlusion was opened, the front teeth was received within correction, the occlusion was finely adjusted to canine fossa staggered relationship and the overbite cover was proper. Results The face type facial soft tissue profile was changed from raised face before treatment to straight face type and 1/3 of height under the face was increased and the temporomandibular disorder was made better. Conclusion the molars occlusion relationship of adult Class Ⅱ Division Ⅰ malocclusion patients accompanied with temporomandibular disorder was adjusted by opening the occlusion and adducting the upright front teeth , the condylar process was moved forward , the posterior teeth alveolar bone height was increased and the temporomandibular disorder was substantially improved.
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Secondary hyperparathyroidism (SHPT) is the common complication in chronic kidney disease patients,especially in those with hemodialysis,which is associated with cardiovascular events and mortality.Active vitamin D and its analogues are classic treatment for SHPT,but some patients are resistant to active vitamin D,and ablation would be a choice for such cases.The purpose of this review is to provide current progress in ablation for SHPT.
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OBJECTIVE@#To analyze external factors affecting auditory ability of infants and toddlers after cochlear implantation in the first year of switch-on.@*METHOD@#Seventy-five infants and toddlers after cochlear implantation were selected as subjects, using LittlEARS Auditory Questionnaire to assess and analyze the correlations with auditory ability and external factors (including gender, cochlear implanted age, pre-implant hearing aid fitting, caregivers' education background, household income and rehabilitation modes) in different stages (before switch-on, and 3 months, 6 months, 9 months, 12 months after switch-on).@*RESULT@#The mean scores of LittlEARS were significantly different in cochlear implanted age group, pre-implant hearing aid fitting group and rehabilitation modes group (P 0.05). The correlations with the mean scores of LittlEARS and cochlear implantation age or pre-implant hearing aid fitting were significant at 3 months or 6 months after switch-on(/r/ ≥ 0. 3, P ≥ 0.3, P < 0.01).@*CONCLUSION@#Cochlear implanted age and pre-implant hearing aid fitting were the important factors affecting auditory ability of infants and toddlers after cochlear implantation in the first year of switch-on. The effect of rehabilitation modes on auditory ability of infants and toddlers after cochlear implantation was slow.
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Child, Preschool , Humans , Infant , Age Factors , Cochlear Implantation , Cochlear Implants , Deafness , Rehabilitation , Hearing , Hearing Aids , Surveys and QuestionnairesABSTRACT
<p><b>OBJECTIVE</b>To understand the infantile nocturnal sleep-wake pattern developmental trajectory with Actiwatch, which would benefit the clinical assessment of infantile sleep.</p><p><b>METHOD</b>This study was a longitudinal study conducted between 7 Oct, 2009-30 Oct, 2011 in 10 hospitals of 9 cities of China ( Beijing, Xi'an, Qingdao, Wuhan, Changsha, Chongqing, Huzhou, Xiamen and Liuzhou). Actiwatch was used to track the sleep-wake pattern development trajectory of healthy infants in the first year of life in the home setting. Participating infants were followed up at 10th day and 28th day during the first month, and then monthly from the second to the sixth month after birth, and then at ninth and twelve months of age respectively. Meanwhile, infantile sleep was observed continuously for about 60 hours at each visit. According to the characteristics of repeated measurement data of this study, two-level random effect model was adopted to analyze the trend of infantile nocturnal sleep-wake parameters changing with age, and the gender difference.</p><p><b>RESULT</b>A total of 473 healthy infants were included in this study, among whom 246 (52.0%) were boys, and 227 (48.0%) were girls; 355 (75.1%) infants completed the whole year follow-up survey. With infants' age increasing, the latency of infants' nighttime sleep onset decreased from 66.8 minutes on 10th day to 15.5-18.7 minutes at 6-12 months of age. The number of night wakes also decreased with age, while uninterrupted sleep periods lengthened with age. On the 10th day, there were 3.0 times of nightwaking on average, and the longest continuous sleeping interval lasted for 227.6 minutes on average. At 12-month of age, infants could sleep continuously for 350.9 minutes at most on average, while the number of nightwaking decreased to 1.6 times per night on average. Generally, nighttime sleep efficiency increased from 66.3% on the 10th day to 86.3% at 12-month of age. The differences of sleep-wake patterns between boys and girls presented as boys' nocturnal longest uninterrupted sleep period was 19 minutes shorter(266.6 vs. 285.6 min), and the average nighttime sleep efficiency was 2.2% lower (74.2% vs. 76.4%) compared with girls respectively. And the differences of sleep efficiency between boys and girls reduced gradually along with the growth.</p><p><b>CONCLUSION</b>During the first 6 months after birth, infantile sleep-wake pattern undergo obvious change. The capability of sleep-onset and uninterrupted sleep improved with age, and the sleep efficiency increased.</p>
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Female , Humans , Infant , Infant, Newborn , Male , China , Longitudinal Studies , Sleep , Physiology , Surveys and QuestionnairesABSTRACT
<p><b>OBJECTIVE</b>To observe the long-term safety and performance of Nurotron cochlear implant system. To analyze the auditory and speech rehabilitation characteristic of pediatric deafened patients after cochlear implantation.</p><p><b>METHODS</b>To analyze 3 years results of pediatric deafened patients with Nurotron® Venus™ cochlear implants. From April to June 2011, sixty deaf children received domestic cochlear implant. All subjects were switched on after 3 to 4 weeks of surgery, and were assessed the auditory rehabilitation on 3 months, 12 months, 24 months and 36 months after switching on. To evaluate the rehabilitation results by Aided Hearing Thresholds, Auditory & Verbal Ability Test and IT-MAIS Questionnaire. Meanwhile, the safety and performance of cochlear implant system were followed-up. Before surgery, all the recipients in this study were 12 to 71 months of age with the average hearing threshold higher than 90 dBHL.</p><p><b>RESULTS</b>Sixty cases of deaf children were implanted successfully. All the recipients used their devices every day, and all of their devices worked well. No complication or side effect related to implantation were found. The sound field aided hearing thresholds, vowels, consonants, monosyllabic words and two-syllable words test, as well as Ling's test, and IT-MAIS Questionnaire of all recipients were improved significantly with prolonged use of CI device. The results of grammatical competence, understanding, communication and presentation skills tests also get significantly promotion in 3 years follow up.</p><p><b>CONCLUSIONS</b>With prolonged use of CI device, the hearing and language tests of children recipients could obtain significantly improvement. The Nurotron® Venus™ CI system works safely and effectively.</p>
Subject(s)
Child , Humans , Cochlear Implantation , Cochlear Implants , Deafness , Rehabilitation , Hearing Tests , Language , Speech Perception , Time Factors , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of chronological age and acoustic device in cognitive development of congenital hearing-impaired infants and toddlers, and analyze the correlation of abilities in cognitive development with other factors.</p><p><b>METHODS</b>Depending on chronological age (1 year old group and 2 years old group) and acoustic device (hearing aids and cochlear implantation), locomotor, personal-social, hearing and speech, hand and eye co-ordination, performance tests in Griffith Cognitive Development Scale were used to assess the cognitive development of 80 hearing-impaired infants and toddlers aged 0-2 years, including before intervention (0 month), after intervention (6, 12 months). Datas were analyzed by Repeated Measurements and Pearson Correlation Test.</p><p><b>RESULTS</b>During 1 year hearing intervention and rehabilitation, hearing and speech, performance and cognitive were extremely significant difference for each phase of early intervention (P < 0.01), the development of locomotor, personal-social, hand and eye co-ordination were no significant difference (P > 0.05). Personal-Social in 1 year old group with hearing impairment was much higher than 2 years old group P < 0.05). Hearing and speech in cochlear implanted group with hearing loss was much higher than hearing aids group. Cognitive development was positive correlation with various region development P < 0.01), and was negatively correlated with chronological age (P > 0.05).</p><p><b>CONCLUSIONS</b>Cognitive development is proportional to recovery time. The chronological age of early intervention obviously affect deaf children's cognitive development. The ability of hearing and speech in cochlear implanted children is superior to children with hearing aids in severe and profound hearing impaired children.</p>